A simple and effective strategy for labeling cysteine sulfenic acid in proteins by utilization of β-ketoesters as cleavable probes

Chem Commun (Camb). 2012 Apr 28;48(34):4091-3. doi: 10.1039/c2cc17868k. Epub 2012 Mar 20.

Abstract

β-ketoesters are robust probes for labeling sulfenic acid (-SOH) proteins allowing quantitative cleavage of the tag for improved analysis of the labeled peptides by MS.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Cell Survival
  • Cysteine / analogs & derivatives*
  • Cysteine / analysis
  • Esters / chemistry
  • Ketones / chemistry
  • Mice
  • Models, Molecular
  • NIH 3T3 Cells
  • Proteins / chemistry*
  • Spectrometry, Mass, Electrospray Ionization*
  • Staining and Labeling
  • Sulfenic Acids / analysis*

Substances

  • Esters
  • Ketones
  • Proteins
  • Sulfenic Acids
  • cysteinesulfenic acid
  • Cysteine