A novel DPPH-HPLC method was successfully developed for evaluating the free radical scavenging activity of Cynomorium songaricum Rupr. The analysis was achieved on a YMC C(30) column with the gradient elution by using water and methanol as mobile phase. The intraday and interday precisions were less than 4.36%. The correlation coefficients of the calibration curve for the standard antioxidants exceeded 0.9967. The proposed method was found to be simple, rapid, and sensitive for evaluating the free radical scavenging activity of natural product. Quantitative analyses of the samples showed that the free radical scavenging activity of the samples from different grown sites varied significantly.
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