A genetically encoded copper(I) sensor based on engineered structural distortion of EGFP

Chem Commun (Camb). 2012 Apr 21;48(32):3890-2. doi: 10.1039/c2cc30531c. Epub 2012 Mar 14.

Abstract

We report a genetically encoded fluorescent sensor for in vivo copper(I) imaging based on engineered intramolecular mechanical strain and structural distortion of EGFP.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CHO Cells
  • Copper / analysis*
  • Cricetinae
  • Cricetulus
  • Green Fluorescent Proteins / chemistry*
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • Microscopy, Fluorescence
  • Spectrometry, Fluorescence*

Substances

  • enhanced green fluorescent protein
  • Green Fluorescent Proteins
  • Copper