Isolation of potentially novel Brucella spp. from frogs

Appl Environ Microbiol. 2012 May;78(10):3753-5. doi: 10.1128/AEM.07509-11. Epub 2012 Mar 9.

Abstract

Bacterial isolates from frogs were phenotypically identified as Ochrobactrum anthropi, but 16S rRNA sequencing showed up to 100% identity with Brucella inopinata. Further analysis of recA, omp2a, omp2b, bcsp31, and IS711 and multilocus sequence analysis (MLSA) verified a close relationship with Brucella, suggesting the isolates may actually represent novel members of this growing genus of zoonotic pathogens.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anura / microbiology*
  • Bacterial Typing Techniques
  • Brucella / classification*
  • Brucella / genetics
  • Brucella / isolation & purification*
  • Brucella / physiology
  • Cluster Analysis
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Genes, Bacterial
  • Molecular Sequence Data
  • Multilocus Sequence Typing
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/HE603354
  • GENBANK/HE603355
  • GENBANK/HE603356
  • GENBANK/HE603357
  • GENBANK/HE603358
  • GENBANK/HE603359
  • GENBANK/HE603360
  • GENBANK/HE603361
  • GENBANK/HE663465
  • GENBANK/HE663466
  • GENBANK/HE663467
  • GENBANK/HE663468
  • GENBANK/HE663469
  • GENBANK/HE663470
  • GENBANK/HE663471
  • GENBANK/HE663472
  • GENBANK/HE663473
  • GENBANK/HE663474
  • GENBANK/HE663475
  • GENBANK/HE663476
  • GENBANK/HE663477
  • GENBANK/HE663478
  • GENBANK/HE663479
  • GENBANK/HE663480