Abstract
A gene, alg7D, from Saccharophagus degradans, coding for a putative alginate lyase belonging to the family of polysaccharide lyase-7, was overexpressed in Escherichia coli. The properties of the recombinant Alg7D were characterized. The enzyme endolytically depolymerized alginate by β-elimination into oligo-alginates with degrees of polymerization of 2-5. Its activity was maximal at 50°C and pH 7 and was slightly increased in the presence of Na(+). The K(M), V(max), k(cat), and k(cat)/K(M) values were: 3 mg ml(-1), 6.2 U mg(-1), 1.9 × 10(-2) s(-1), and 6.3 × 10(-3) mg(-1 )ml s(-1), respectively.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alginates / metabolism
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Alteromonadaceae / enzymology*
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Alteromonadaceae / genetics
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Enzyme Stability
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Escherichia coli / genetics
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Gene Expression
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Glucuronic Acid / metabolism
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Hexuronic Acids / metabolism
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Hydrogen-Ion Concentration
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Kinetics
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Oligosaccharides / metabolism
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Polysaccharide-Lyases / chemistry
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Polysaccharide-Lyases / genetics*
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Polysaccharide-Lyases / metabolism*
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Temperature
Substances
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Alginates
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Hexuronic Acids
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Oligosaccharides
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Recombinant Proteins
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Glucuronic Acid
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Polysaccharide-Lyases
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poly(beta-D-mannuronate) lyase