Signaling pathways of immobilized FGF-2 on silicon-substituted hydroxyapatite

Macromol Biosci. 2012 Apr;12(4):446-53. doi: 10.1002/mabi.201100456. Epub 2012 Mar 2.

Abstract

Therapeutic strategies for bone regeneration involve the selection of suitable biomaterials, growth factors, and cell types to mimic the cellular microenvironment where molecular and mechanical signals control the reconstruction of bone tissue. The immobilization of basic fibroblast growth factor (FGF-2) on powdered silicon-substituted hydroxyapatite (Si-HA) allows to prepare a biofunctional biomaterial able to interact with bone cells in a very specific way. The biological activity of FGF-2/Si-HA, evaluated in Saos-2 osteoblasts and MC3T3-E1 preosteoblasts through the PLCγ and MAPK/ERK signal transduction pathways, shows that FGF-2 immobilized on Si-HA provides the right signals to cells stimulating crucial intracellular mechanisms of osteoblast proliferation and differentiation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis / drug effects
  • Biomimetic Materials / chemical synthesis*
  • Biomimetic Materials / pharmacology
  • Bone and Bones / cytology
  • Bone and Bones / drug effects
  • Cell Cycle / drug effects
  • Cell Differentiation / drug effects
  • Cell Line
  • Cell Proliferation / drug effects
  • Durapatite / chemistry*
  • Fibroblast Growth Factor 2 / chemistry
  • Fibroblast Growth Factor 2 / metabolism*
  • Humans
  • Immobilized Proteins
  • Mice
  • Mitogen-Activated Protein Kinase Kinases / metabolism
  • Osteoblasts / cytology
  • Osteoblasts / drug effects*
  • Osteoblasts / metabolism
  • Phospholipase C gamma / metabolism
  • Powders
  • Signal Transduction / physiology*
  • Silicon / chemistry*
  • Tissue Engineering / methods

Substances

  • Immobilized Proteins
  • Powders
  • Fibroblast Growth Factor 2
  • Durapatite
  • Mitogen-Activated Protein Kinase Kinases
  • Phospholipase C gamma
  • Silicon