Abstract
Universal phenotyping techniques that can discriminate among various states of biological systems have great potential. We applied 557 fluorescent library compounds to NCI's 60 human cancer cell-lines (NCI-60) to generate a systematic fluorescence phenotypic profiling data. By the kinetic fluorescence intensity analysis, we successfully discriminated the organ origin of all the 60 cell-lines.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Boron Compounds / pharmacology
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Cell Line, Tumor
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Cluster Analysis
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Fluorescence
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Fluorescent Dyes / chemistry
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Gene Expression Profiling
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Gene Expression Regulation, Neoplastic*
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Humans
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Kinetics
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Microscopy, Fluorescence / methods
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Organic Chemicals / pharmacology
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Phenotype
Substances
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4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
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Boron Compounds
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Fluorescent Dyes
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Organic Chemicals
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red dye CMXRos