High-throughput method for a kinetics analysis of the high-pressure inactivation of microorganisms using microplates

Toshimi Hasegawa; Manabu Hayashi; Kazuki Nomura; Mayumi Hayashi; Miyuki Kido; Tsuneo Ohmori; Masao Fukuda; Akinori Iguchi; Shigeaki Ueno; Toru Shigematsu; Masao Hirayama; Tomoyuki Fujii

doi:10.1016/j.jbiosc.2012.02.001

High-throughput method for a kinetics analysis of the high-pressure inactivation of microorganisms using microplates

J Biosci Bioeng. 2012 Jun;113(6):788-91. doi: 10.1016/j.jbiosc.2012.02.001. Epub 2012 Feb 29.

Authors

Toshimi Hasegawa¹, Manabu Hayashi, Kazuki Nomura, Mayumi Hayashi, Miyuki Kido, Tsuneo Ohmori, Masao Fukuda, Akinori Iguchi, Shigeaki Ueno, Toru Shigematsu, Masao Hirayama, Tomoyuki Fujii

Affiliation

¹ Department of Food Science, Faculty of Applied Life Sciences, Niigata University of Pharmacy and Applied Life Sciences, 265-1 Higashijima, Akiha-ku, Niigata, Niigata 956-8603, Japan.

PMID: 22382011
DOI: 10.1016/j.jbiosc.2012.02.001

Abstract

Using microplates as pressure and cultivation vessels, a high-throughput method was developed for analyzing the high-pressure inactivation kinetics of microorganisms. The loss of viability from a high-pressure treatment, measured based on the growth delay during microplate cultivation, showed reproducibility with the conventional agar plate method and was applicable for the kinetics analysis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Disinfection / methods*
Kinetics
Microbial Viability*
Pressure*
Reproducibility of Results