Objective: To study the aqueous two-phase system which could be used for extraction of berberine hydrochloride.
Methods: Three aqueous two-phase systems were used for extraction experiment firstly, the best one was chosen from that, and then the single factor experiment and orthogonal experiment were carried out.
Results: 10 mL 95% ethanol with 10 mL 2.2 mol/L ammonium sulfate could make a aqueous two-phase system, added 600 mg berberine hydrochloride whose purity was 53.22% into it, regulated its pH to 4, then the system was put into water-bath heater (70 degrees C) for 30 min, the extraction rate could reach 99.29%; Collected the extraction liquid, dried it under 40 degrees C, the purity of berberine hydrochloride was 88.43%.
Conclusion: This system is a suitable aqueous two-phase system for extraction of berberine hydrochloride.