Abstract
This paper describes an approach called ensemble decision aliquot ranking (eDAR) for isolating rare cells from peripheral blood. eDAR has a recovery of over 93% (n=9) with a zero false positive rate (n=8), and provides direct easy access to individual isolated live cells for downstream single-cell manipulation and analysis. We anticipate eDAR will enable new studies of various types of rare cells that circulate in blood.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies / immunology
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Antigens, Neoplasm / immunology
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Antigens, Neoplasm / metabolism
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Blood Cells / cytology*
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Cell Adhesion Molecules / immunology
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Cell Adhesion Molecules / metabolism
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Epithelial Cell Adhesion Molecule
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Flow Cytometry
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Fluorescent Dyes / chemistry
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High-Throughput Screening Assays
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Humans
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Microscopy, Confocal
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Neoplastic Cells, Circulating*
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Neoplastic Stem Cells / cytology
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Neoplastic Stem Cells / metabolism
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Receptor, ErbB-2 / immunology
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Receptor, ErbB-2 / metabolism
Substances
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Antibodies
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Antigens, Neoplasm
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Cell Adhesion Molecules
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Epithelial Cell Adhesion Molecule
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Fluorescent Dyes
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ERBB2 protein, human
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Receptor, ErbB-2