The optimization of serum-free medium for the production of the scu-PA by the addition of algal extracts

H G Kim; K D Sung; M S Ham; K H Chung; K H Chung; H Y Lee

doi:10.1007/BF00749654

The optimization of serum-free medium for the production of the scu-PA by the addition of algal extracts

Cytotechnology. 1995 Oct;17(3):165-72. doi: 10.1007/BF00749654.

Authors

H G Kim¹, K D Sung, M S Ham, K H Chung, K H Chung, H Y Lee

Affiliation

¹ Department of Food Engineering, Kangwon National University, 200-701, Chunchon, S. Korea.

PMID: 22358556
DOI: 10.1007/BF00749654

Abstract

The addition of 2.8 μg/ml algal extracts enhanced both scu-PA production and cell growth in a serum-free medium, compared to a conventional serum-free medium for the cultivation of recombinant CHO cells. The growth rate and scu-PA production were relatively lower in the serum-free medium than 5% serum containing medium: however, specific scu-PA production rate was higher in the serum-free medium due to the long-term period of cultivation (3.66×10(-4) vs. 2.48×10(-4) IU/cell/day). Overall scu-PA production rate was also greater in an enforced serum-free medium as 25,000 IU/day over 50 d of perfusion cultivation. The conversion ratio of scu-PA to tcu-PA was greatly reduced in the serum-free medium during perfusion cultivation (10% compared to 20% conversion in a serum containing medium).