Blood Drawing has dramatically improved medical treatment and medical examination in the 20th century. The system of evacuated blood collection tube was invented in the US mainly to improve accuracy and efficiency of clinical examination while improving Health Care Workers safety (reduce shapes injuries) in 1970's. After the new method of blood collection spread throughout the world, laboratory automation systems and reagents have also improved. In present time, blood is the most important sample for medical examination and treatment. There are various blood collection guidelines Clinical and Laboratory Standards Institute; CLSI, the Japanese Committee for Clinical Laboratory Standards; JCCLS and the Brazilian Society of Clinical Pathology/Laboratory Medicine; BCP/LM, textbooks and training system in the world. Learning the best practice for blood collection technique is crucial in order to reduce pre-analytical error and provide accurate clinical data. Therefore we have to take not only a technical training, but also obtain information from either internal or external sources for developing our knowledge for improving our blood drawing technique.