Exposure of the dorsal root ganglion to pulsed radiofrequency current in a neuropathic pain model of peripheral nerve injury

Danielle Perret; Doo-Sik Kim; Kang-Wu Li; Z David Luo

doi:10.1007/978-1-61779-561-9_21

Exposure of the dorsal root ganglion to pulsed radiofrequency current in a neuropathic pain model of peripheral nerve injury

Methods Mol Biol. 2012:851:275-84. doi: 10.1007/978-1-61779-561-9_21.

Authors

Danielle Perret¹, Doo-Sik Kim, Kang-Wu Li, Z David Luo

Affiliation

¹ Department of Anesthesiology & Perioperative Care, School of Medicine, University of California Irvine, Irvine, CA, USA.

PMID: 22351099
DOI: 10.1007/978-1-61779-561-9_21

Abstract

The spinal nerve ligation model of neuropathic pain in rats, as originally described by Kim and Chung (Pain 50:355-363, 1992), provides an excellent venue to study the antinociception and modulation effects of pulsed radiofrequency (PRF) current in pain processing. We describe the procedure of application of PRF current near the exposed L5 dorsal root ganglion (DRG) in rats with L5 spinal nerve ligation injury-induced behavioral hypersensitivity. This method employs the direct visualization of the L5 DRG, allowing for confirmation of the location of the PRF probe adjacent to the DRG.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Disease Models, Animal
Ganglia, Spinal / pathology
Ganglia, Spinal / physiopathology
Ganglia, Spinal / radiation effects*
Neuralgia / etiology*
Neuralgia / pathology
Pain Measurement
Peripheral Nerve Injuries / complications*
Peripheral Nerve Injuries / pathology
Radio Waves
Rats
Rats, Sprague-Dawley