Isolation of ubiquitylated proteins using tandem ubiquitin-binding entities

Methods Mol Biol. 2012:832:173-83. doi: 10.1007/978-1-61779-474-2_12.

Abstract

Studying postubiquitylation events has always been a difficult task due to the labile nature of these posttranslational modifications. When utilized in tandem, ubiquitin-binding entities (TUBEs) not only increase up to thousand times the affinity for poly-ubiquitin chains but also protect ubiquitylated proteins from the action of the proteasome and de-ubiquitylating enzymes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, Affinity / methods*
  • Polyubiquitin / chemistry*
  • Polyubiquitin / isolation & purification*
  • Polyubiquitin / metabolism
  • Protein Binding
  • Protein Processing, Post-Translational
  • Protein Structure, Tertiary
  • Ubiquitination

Substances

  • Polyubiquitin