Real-time multiplex PCR for detecting Shiga toxin 2-producing Escherichia coli O104:H4 in human stools

Wenlan Zhang; Martina Bielaszewska; Andreas Bauwens; Angelika Fruth; Alexander Mellmann; Helge Karch

doi:10.1128/JCM.06817-11

Real-time multiplex PCR for detecting Shiga toxin 2-producing Escherichia coli O104:H4 in human stools

J Clin Microbiol. 2012 May;50(5):1752-4. doi: 10.1128/JCM.06817-11. Epub 2012 Feb 15.

Authors

Wenlan Zhang¹, Martina Bielaszewska, Andreas Bauwens, Angelika Fruth, Alexander Mellmann, Helge Karch

Affiliation

¹ Institute for Hygiene and the National Consulting Laboratory for Hemolytic Uremic Syndrome, University of Münster, Münster, Germany.

Abstract

A real-time multiplex PCR targeting stx(2), wzy(O104), and fliC(H4) of enterohemorrhagic Escherichia coli (EHEC) O104:H4 correctly determined the presence or absence of these genes in 253 EHEC isolates and enrichment cultures of stool samples from 132 patients. It is a rapid, sensitive, and specific tool for detecting EHEC O104:H4 in human stools.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Bacteriological Techniques / methods*
Enterohemorrhagic Escherichia coli / genetics
Enterohemorrhagic Escherichia coli / isolation & purification*
Enterohemorrhagic Escherichia coli / pathogenicity
Escherichia coli Infections / microbiology*
Feces / microbiology*
Humans
Multiplex Polymerase Chain Reaction / methods*
Real-Time Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Shiga Toxin 2 / genetics*
Time Factors

Substances

Shiga Toxin 2