Synchrotron radiation FTIR detection of a metal-carbonyl tamoxifen analog. Correlation with luminescence microscopy to study its subcellular distribution

Biotechnol Adv. 2013 May-Jun;31(3):393-5. doi: 10.1016/j.biotechadv.2012.01.023. Epub 2012 Jan 31.

Abstract

1,1-Di(4-hydroxyphenyl)-2-cyrhetrenylbut-1-ene 1 is an organometallic conjugate where a [(Cp)Re(CO)(3)] unit is linked to a hydroxytamoxifen-like structure. Its subcellular nuclear distribution was previously observed in a single cell using the near-field technique AFMIR. We show here that synchrotron radiation FTIR spectromicroscopy (SR-FTIR-SM) enabled the mapping of 1 based on its IR-signature (characteristic bands in the 1850-2200 cm(-1) range) and pointed out the colocalization of 1 with an area of high amide density. Fluorescence microscopy using DAPI staining performed on the same cells confirmed that this area corresponds to the cell nucleus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Breast Neoplasms / metabolism
  • Cell Line, Tumor / metabolism
  • Cell Nucleus / metabolism
  • Female
  • Humans
  • Microscopy, Fluorescence / methods*
  • Organometallic Compounds / analysis*
  • Organometallic Compounds / pharmacokinetics
  • Phenols / analysis*
  • Phenols / pharmacokinetics
  • Rhenium / analysis
  • Spectrophotometry, Infrared / methods
  • Spectroscopy, Fourier Transform Infrared / methods*
  • Synchrotrons
  • Tamoxifen / analogs & derivatives*

Substances

  • Organometallic Compounds
  • Phenols
  • Tamoxifen
  • Rhenium