Abstract
Three new cycloartenol triterpene saponins, 3 β,16 α-dihydroxy-12-acetoxy-16,22-cyclo-23-ketone-24 R,25-epoxy-cycloartane-7-ene 3- O- β-D-galactopyranoside ( 1), 24- O-hydroxy-7,8-didehydrohydroshengmanol 3- O- β-D-galactopyranoside ( 2), and 24-epi-24- O-hydroxy-7,8-didehydrohydroshengmanol 3- O- β-D-galactopyranoside ( 3), were isolated from the ethyl acetate soluble fraction of Cimicifuga simplex. Their structures were established by detailed spectroscopic analysis, including extensive 2D-NMR data. This is the first time that a 16,22-cyclo type glycosidesaponin from aCimicifuga species was reported. The immunosuppressive activities of the new compounds were evaluated by a ConA-stimulated T splenocyte proliferation assay in vitro.
© Georg Thieme Verlag KG Stuttgart · New York.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Line, Tumor
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Cell Proliferation
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Cimicifuga / chemistry*
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Glycosides / chemistry
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Glycosides / isolation & purification
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Glycosides / pharmacology*
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Humans
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Immunosuppressive Agents / chemistry
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Immunosuppressive Agents / isolation & purification
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Immunosuppressive Agents / pharmacology*
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Lymphocyte Activation / drug effects
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Magnetic Resonance Spectroscopy
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Molecular Structure
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Phytosterols / chemistry
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Phytosterols / isolation & purification
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Phytosterols / pharmacology*
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Plant Extracts / chemistry
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Plant Extracts / isolation & purification
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Plant Roots / chemistry
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Spleen / cytology
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T-Lymphocytes / cytology
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T-Lymphocytes / drug effects*
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Triterpenes / chemistry
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Triterpenes / isolation & purification
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Triterpenes / pharmacology*
Substances
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24-O-hydroxy-7,8-didehydrohydroshengmanol-3-O-beta-D-galactopyranoside
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24-epi-24-O-hydroxy-7,8-didehydrohydroshengmanol-3-D-beta-D-galactopyranoside
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3beta,16alpha-dihydroxy-12-acetoxy-16,22-cyclo-23-ketone-24 R,25-epoxy-cycloartane-7-ene 3-O- beta-D-galactopyranoside
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Glycosides
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Immunosuppressive Agents
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Phytosterols
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Plant Extracts
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Triterpenes
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cycloartenol