The goal of this study was to examine the pathophysiological changes to erythrocytes in multiple myeloma (MM) patients at a nanometer scale. We hypothesize that studying changes in red blood cells may be important for early diagnosis and effective treatment of MM. Blood samples were taken from ten healthy volunteers and ten MM patients before and after treatment. Changes in the morphological and biomechanical properties of the erythrocytes were studied at a nanometer scale with atomic force microscopy (AFM). There were dramatic deformations in the overall shape and surface membrane of the erythrocytes from pre- and post therapeutic MM patients compared with the healthy controls. Healthy and pathological MM erythrocytes could be distinguished by several morphologic parameters, including the width, length, length to width ratio, valley, peak, valley-to-peak, standard deviation, and surface fluctuation. The effectiveness of disease treatment could also be evaluated by studying these red blood cell parameters. AFM was able to detect noticeable morphological differences in the red blood cells from MM patients compared with healthy controls. Therefore, erythrocyte morphology is an important parameter for diagnosing MM, as well as evaluating the efficacy of disease treatment.
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