Trichinella spiralis infection induces β-actin co-localized with thymosin β4

Abstract

Trichinella spiralis (T. spiralis) infection in muscle is characterized by the vascular network for the nurse cell-larva complex. We showed in a previous report that thymosin β4 was up-regulated during nurse cell formation by T. spiralis. As thymosin β4 (Tβ4) is the actin-sequestering protein that regulates actin polymerization, the expression pattern of β-actin during the nurse cell formation was analyzed. The protein level of β-actin in muscle fibers 10 days after infection was significantly increased, and its expression remained high in the nurse cells for six weeks. In order to investigate the functional relationship between Tβ4 and β-actin, localization of two proteins was analyzed. Immunofluorescence showed that Tβ4 and β-actin were co-localized in the T. spiralis-infected nurse cells from 10 days to six weeks. The expression patterns of other actin-binding proteins, including thymosin β10 (Tβ10), subunits of the Arp2/3 complex, subunits of Capping protein, profilin, and cofilin, were also analyzed at the mRNA level. Tβ10 expression was also increased during nurse cell formation. Expressions of the Arp2/3 complex was increased at 21 days after infection and Capping proteins was increased during nurse cell formation but shows different expression patterns, depending on the subunit. Profilin and cofilin were specifically increased in the muscle fibers from 14 days after infection. These data show that Tβ4 and β-actin are over-expressed during nurse cell formation upon T. spiralis infection and may be involved in nurse cell formation along with other actin-binding proteins.