A versatile platform for highly sensitive detection of protein: DNA enriching magnetic nanoparticles based rolling circle amplification immunoassay

Chem Commun (Camb). 2012 Apr 25;48(33):3930-2. doi: 10.1039/c2cc18007c. Epub 2012 Feb 3.

Abstract

A novel rolling circle amplification (RCA) immunoassay based on DNA enriching magnetic nanoparticles and assembled fluorescent DNA nanotags, magnetic nanoparticles-RCA immunoassay, is developed as a versatile fluorescence assay platform for highly sensitive proteins detection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biosensing Techniques / methods*
  • DNA / chemistry*
  • DNA / genetics
  • Humans
  • Immunoassay / methods*
  • Immunoglobulin G / analysis
  • Immunoglobulin G / chemistry
  • Magnets / chemistry*
  • Mice
  • Nanoparticles / chemistry*
  • Nucleic Acid Amplification Techniques / methods*
  • Proteins / analysis*
  • Proteins / chemistry

Substances

  • Immunoglobulin G
  • Proteins
  • DNA