Abstract
Staphylococcus (S.) aureus is a major pathogen involved in chronic bovine mastitis. Staphylococcal mastitis is difficult to control due to the ability of S. aureus to invade and survive within host cells. We therefore postulated that induction of CD8(+) cytotoxic T lymphocyte (CTL) responses leading to destruction of infected cells could help in the control of S. aureus mastitis. We demonstrate that immunization of mice with heat-killed S. aureus together with agonistic anti-CD40 monoclonal antibodies elicits strong CTL responses capable of reducing the severity of subsequent staphylococcal mastitis. Our study shows promise for CTL-dependent vaccination against S. aureus mastitis.
Copyright © 2012 Elsevier Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Monoclonal / administration & dosage
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Antibodies, Monoclonal / immunology
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CD40 Antigens / immunology*
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CD8-Positive T-Lymphocytes / immunology*
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Disease Models, Animal
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Mastitis / immunology
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Mastitis / pathology
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Mastitis / prevention & control*
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C57BL
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Staphylococcal Infections / immunology
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Staphylococcal Infections / pathology
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Staphylococcal Infections / prevention & control*
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Staphylococcal Vaccines / administration & dosage
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Staphylococcal Vaccines / immunology*
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Staphylococcus aureus / immunology*
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Staphylococcus aureus / pathogenicity
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T-Lymphocytes, Cytotoxic / immunology
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Vaccines, Inactivated / administration & dosage
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Vaccines, Inactivated / immunology
Substances
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Antibodies, Monoclonal
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CD40 Antigens
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Staphylococcal Vaccines
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Vaccines, Inactivated