Abstract
A single-cycle, propagation-defective replicon particle (RP) vaccine expressing a swine influenza virus hemagglutinin (HA) gene was constructed and evaluated in several different animal studies. Studies done in both the intended host (pigs) and non-host (mice) species demonstrated that the RP vaccine is not shed or spread by vaccinated animals to comingled cohorts, nor does it revert to virulence following vaccination. In addition, vaccinated pigs develop both specific humoral and IFN-γ immune responses, and young pigs are protected against homologous influenza virus challenge.
Copyright © 2012 Elsevier Ltd. All rights reserved.
MeSH terms
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Alphavirus / genetics
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Alphavirus / immunology*
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Animals
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Antibodies, Viral / blood
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Antibody Formation
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Cytopathogenic Effect, Viral
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Female
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Hemagglutinin Glycoproteins, Influenza Virus / genetics
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Hemagglutinin Glycoproteins, Influenza Virus / immunology*
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Influenza Vaccines / genetics
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Influenza Vaccines / immunology*
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Interferon-gamma / immunology
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Male
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Mice
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Mice, Inbred BALB C
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Orthomyxoviridae / immunology
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Orthomyxoviridae / pathogenicity
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Orthomyxoviridae Infections / immunology
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Orthomyxoviridae Infections / prevention & control*
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Replicon*
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Swine / immunology
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Virulence
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Virus Shedding
Substances
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Antibodies, Viral
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Hemagglutinin Glycoproteins, Influenza Virus
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Influenza Vaccines
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Interferon-gamma