Incidence of Epstein-Barr virus in pediatric leukemia in the Sudan

Abstract

Background: Results of several studies have suggested a probable etiologic association between Epstein-Barr virus (EBV) and leukemias; therefore, the aim of this study was to investigate the association of EBV in childhood leukemia.

Methods: A direct isothermal amplification method was developed for detection of the latent membrane protein 1 (LMP1) of EBV in the peripheral blood of 80 patients with leukemia (54 had lymphoid leukemia and 26 had myeloid leukemia) and of 20 hematologically healthy control subjects.

Results: EBV LMP1 gene transcripts were found in 29 (36.3%) of the 80 patients with leukemia but in none of the healthy controls (P < .0001). Of the 29 EBV(+) cases, 23 (79.3%), 5 (17.3%), and 1 (3.4%) were acute lymphoblastic leukemia, acute myeloid leukemia, and chronic myeloid leukemia, respectively.

Conclusion: EBV LMP1 gene transcriptional activity was observed in a significant proportion of patients with acute lymphoblastic leukemia. EBV infection in patients with lymphoid leukemia may be a factor involved in the high incidence of pediatric leukemia in the Sudan.