Key role of CXCL13/CXCR5 axis for cerebrospinal fluid B cell recruitment in pediatric OMS

Michael R Pranzatelli; Elizabeth D Tate; Nathan R McGee; Anna L Travelstead; Richard M Ransohoff; Jayne M Ness; Jerry A Colliver

doi:10.1016/j.jneuroim.2011.12.014

Key role of CXCL13/CXCR5 axis for cerebrospinal fluid B cell recruitment in pediatric OMS

J Neuroimmunol. 2012 Feb 29;243(1-2):81-8. doi: 10.1016/j.jneuroim.2011.12.014. Epub 2012 Jan 20.

Authors

Michael R Pranzatelli¹, Elizabeth D Tate, Nathan R McGee, Anna L Travelstead, Richard M Ransohoff, Jayne M Ness, Jerry A Colliver

Affiliation

¹ National Pediatric Myoclonus Center and Neuroimmunology Laboratory, Southern Illinois University School of Medicine, PO Box 19643, Springfield, IL 62794-9643, USA. mpranzatelli@siumed.edu

PMID: 22264765
DOI: 10.1016/j.jneuroim.2011.12.014

Abstract

To study aberrant B cell trafficking into the CSF in opsoclonus-myoclonus syndrome (OMS), chemoattractants CXCL13 and CXCL12, and B cell frequency and CXCR5 expression, were evaluated. CSF CXCL13 concentration and the CSF/serum ratio were higher in untreated OMS than controls, related directly to OMS severity and inversely to OMS duration, and correlated with CSF B cell frequency and oligoclonal bands. CXCL12 showed the opposite pattern. Selective accumulation of CXCR5+ memory B cells in CSF was found. In ACTH-treated OMS, CXCL13, but not CXCL12, was lower. These data implicate the chemokine/chemoreceptor pair CXCL13/CXR5 in B cell recruitment to the CNS in OMS. CXCL13 and CXCL12 may serve as reciprocal biomarkers of disease activity, but CXCL13 also had utility as a treatment biomarker.

Trial registration: ClinicalTrials.gov NCT00806182.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adolescent
Adrenal Cortex Hormones / therapeutic use
Adrenocorticotropic Hormone / pharmacology
Adrenocorticotropic Hormone / therapeutic use
Analysis of Variance
Antigens, CD / metabolism
B-Lymphocytes / drug effects
B-Lymphocytes / metabolism*
Case-Control Studies
Cell Movement / drug effects
Chemokine CXCL12 / cerebrospinal fluid
Chemokine CXCL12 / metabolism
Chemokine CXCL13 / cerebrospinal fluid
Chemokine CXCL13 / metabolism*
Child
Child, Preschool
Cross-Sectional Studies
Enzyme-Linked Immunosorbent Assay
Female
Flow Cytometry
Humans
Immunoglobulins, Intravenous / therapeutic use
Infant
Longitudinal Studies
Male
Opsoclonus-Myoclonus Syndrome / cerebrospinal fluid*
Opsoclonus-Myoclonus Syndrome / drug therapy
Receptors, CXCR5 / metabolism*

Substances

Adrenal Cortex Hormones
Antigens, CD
CXCL12 protein, human
CXCL13 protein, human
CXCR5 protein, human
Chemokine CXCL12
Chemokine CXCL13
Immunoglobulins, Intravenous
Receptors, CXCR5
Adrenocorticotropic Hormone

Associated data

ClinicalTrials.gov/NCT00806182