Objective: To prepare Standard Operating Procedure (SOP) for the artificial breeding technology of Poecilobdella manillensi.
Methods: The environment of producing area and breeding technology, the control of diseases, reserving seeds for breeding, harvesting and processing,the standard of the product quality and supervision for the standardization breeding of Poecilobdella Manillensis was studied.
Results: The average yield of vital specimen of Poecilobdella Manillensis was 420.88 kg/mu, the natural hirudin in 1kg of the living specimen of Poecilobdella Manillensis contained 430,000 AT-U, and the quality index and hygienic standard of all products complied with the regulations and standards of the State.
Conclusion: SOP is applicable for the breeding of Poecilobdella manillensi for Guangxi district.