Detection of tip-enhanced fluorescence from loop-mediated isothermal amplification of hepatitis B virus by two-photon microscopy

Shih-Chung Wei; Tsung-Liang Chuang; Hui-Hsin Lu; Chia-Chen Chang; Da-Shin Wang; Kung-Bin Sung; Chii-Wann Lin

doi:10.1109/IEMBS.2011.6090087

Detection of tip-enhanced fluorescence from loop-mediated isothermal amplification of hepatitis B virus by two-photon microscopy

Annu Int Conf IEEE Eng Med Biol Soc. 2011:2011:490-3. doi: 10.1109/IEMBS.2011.6090087.

Authors

Shih-Chung Wei¹, Tsung-Liang Chuang, Hui-Hsin Lu, Chia-Chen Chang, Da-Shin Wang, Kung-Bin Sung, Chii-Wann Lin

Affiliation

¹ Institute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taiwan.

PMID: 22254355
DOI: 10.1109/IEMBS.2011.6090087

Abstract

Tip-enhanced fluorescence of localized DNA replication by loop-mediated isothermal amplification (LAMP) is a potential way to observe real-time biological reaction confined in nanometer scale. We successfully coated Bst polymerase on the apex (~100 nm) of an atomic force microscope (AFM) tip and performed localized LAMP reaction of hepatitis B virus (HBV). By using this tip-based reaction, the replicated HBV DNA can be directly imaged to be 400~500 nm spots by using two-photon excitation fluorescence microscopy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA Probes / genetics
DNA Probes / ultrastructure
DNA, Viral / genetics*
DNA, Viral / ultrastructure
Hepatitis B virus / genetics*
Hepatitis B virus / ultrastructure
Image Enhancement / methods*
Microscopy, Fluorescence, Multiphoton / methods*
Molecular Imaging / methods*
Molecular Probe Techniques*
Nucleic Acid Amplification Techniques / methods*

Substances

DNA Probes
DNA, Viral