Dead-end hollow-fiber ultrafiltration for concentration and enumeration of Escherichia coli and broad-host-range plasmid DNA from wastewater

J Microbiol Methods. 2012 Mar;88(3):430-2. doi: 10.1016/j.mimet.2012.01.005. Epub 2012 Jan 9.

Abstract

Broad-host-range plasmids can facilitate dissemination of antibiotic resistance determinants among diverse bacterial populations. We evaluated hollow-fiber ultrafiltration for increases in detection efficiency of broad-host-range plasmids and Escherichia coli DNA in wastewater. Ultrafiltration followed by PCR showed limited increases in DNA detection and quantification in effluent compared with membrane filtration alone.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA, Bacterial / analysis
  • DNA, Bacterial / genetics
  • DNA, Bacterial / isolation & purification*
  • Escherichia coli / genetics*
  • Plasmids / analysis
  • Plasmids / genetics
  • Plasmids / isolation & purification*
  • Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Ultrafiltration / methods*

Substances

  • DNA, Bacterial