Amphibians are commonly kept in laboratory and zoological facilities and are becoming more frequent as pets. However, many amphibian species are declining in the wild owing to a variety of infectious and noninfectious diseases. This article reviews the current state of knowledge of mycobacteriosis in amphibian species, including pathogenesis, clinical signs, appropriate diagnostics, treatment options, and zoonotic potential and prevention. It is hoped this review will provide clinical veterinarians and scientists the tools they need to provide better care for amphibian species suffering mycobacteriosis, as well as serve to stimulate additional research into amphibians affected by mycobacterosis.