Functional characterization of CP148, a novel key component for centrosome integrity in Dictyostelium

Cell Mol Life Sci. 2012 Jun;69(11):1875-88. doi: 10.1007/s00018-011-0904-2. Epub 2012 Jan 6.

Abstract

The Dictyostelium centrosome consists of a layered core structure surrounded by a microtubule-nucleating corona. A tight linkage through the nuclear envelope connects the cytosolic centrosome with the clustered centromeres within the nuclear matrix. At G2/M the corona dissociates, and the core structure duplicates, yielding two spindle poles. CP148 is a novel coiled coil protein of the centrosomal corona. GFP-CP148 exhibited cell cycle-dependent presence and absence at the centrosome, which correlates with dissociation of the corona in prophase and its reformation in late telophase. During telophase, GFP-CP148 formed cytosolic foci, which coalesced and joined the centrosome. This explains the hypertrophic appearance of the corona upon strong overexpression of GFP-CP148. Depletion of CP148 by RNAi caused virtual loss of the corona and disorganization of interphase microtubules. Surprisingly, formation of the mitotic spindle and astral microtubules was unaffected. Thus, microtubule nucleation complexes associate with centrosomal core components through different means during interphase and mitosis. Furthermore, CP148 RNAi caused dispersal of centromeres and altered Sun1 distribution at the nuclear envelope, suggesting a role of CP148 in the linkage between centrosomes and centromeres. Taken together, CP148 is an essential factor for the formation of the centrosomal corona, which in turn is required for centrosome/centromere linkage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Cycle
  • Centrosome / metabolism
  • Centrosome / ultrastructure*
  • Dictyostelium / genetics
  • Dictyostelium / metabolism
  • Dictyostelium / ultrastructure*
  • Green Fluorescent Proteins / analysis
  • Microtubules / metabolism
  • Microtubules / ultrastructure
  • Protozoan Proteins / analysis
  • Protozoan Proteins / antagonists & inhibitors
  • Protozoan Proteins / physiology*
  • RNA Interference

Substances

  • Protozoan Proteins
  • Green Fluorescent Proteins