A sensitive and specific HPLC-UV method was developed and validated for the determination of xyloketal B in rat plasma. Following liquid-liquid extraction, the separation was performed using an isocratic mobile phase of methanol-acetonitrile-water (30/30/40, v/v/v) on a Phenomenex C(18) column (4.6mm×250mm, 5μm). The eluent was monitored at 220nm and at a flow rate of 0.8mlmin(-1). A linear curve over the concentration range of 1-128μg/ml (r>0.999) was established. The LLOQ of the method was 1μg/ml. Good precision and accuracy at concentrations of 2.5, 25 and 100μg/ml were obtained. The recovery of xyloketal B in plasma was >87.91%. The validated method was found to be specific, precise and accurate in the study. The analytic method was satisfactorily applied to perform preclinical pharmacokinetic study of xyloketal B in rat plasma.
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