Colorimetric tracking of protein structural evolution based on the distance-dependent light scattering of embedded gold nanoparticles

Inhee Choi; Young In Yang; Eunhye Jeong; Kihoon Kim; Surin Hong; Taewook Kang; Jongheop Yi

doi:10.1039/c2cc17174k

Colorimetric tracking of protein structural evolution based on the distance-dependent light scattering of embedded gold nanoparticles

Chem Commun (Camb). 2012 Feb 25;48(17):2286-8. doi: 10.1039/c2cc17174k. Epub 2012 Jan 4.

Authors

Inhee Choi¹, Young In Yang, Eunhye Jeong, Kihoon Kim, Surin Hong, Taewook Kang, Jongheop Yi

Affiliation

¹ School of Chemical and Biological Engineering, Institute of Chemical Processes, Seoul National University, Seoul, 151-742, Korea.

PMID: 22218614
DOI: 10.1039/c2cc17174k

Abstract

In this communication, we describe a new, simplified colorimetric method for in situ tracking of structural evolution of Cu/Zn-superoxide dismutase (SOD1) aggregates, based on changes in plasmonic coupling between gold nanoparticles (GNPs) embedded along the structural backbone of the SOD1 aggregates.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Colorimetry
Gold / chemistry*
Light
Metal Nanoparticles / chemistry*
Microscopy, Atomic Force
Scattering, Radiation
Superoxide Dismutase / chemistry*
Superoxide Dismutase / metabolism
Superoxide Dismutase-1

Substances

Gold
Superoxide Dismutase
Superoxide Dismutase-1