Abstract
The plant hypocotyl is an excellent model for the analysis of cell elongation. We have characterized a knockout mutant of the Arabidopsis TIM50 gene that showed a reduction in the hypocotyls length of etiolated seedlings. We also found that a knockout of TIM50 caused enlargement and deformation of the mitochondrial structure and a reduction in intracellular ATP levels. TIM50 is a component of the mitochondrial TIM23 inner membrane protein complex and is involved in the import of mitochondrial proteins. The short hypocotyl phenotype was recovered by the addition of Compound C, an inhibitor of AMPK. Thus, the mitochondrial ATP level controls cell elongation in Arabidopsis hypocotyls through possible signaling via AMPK.
Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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AMP-Activated Protein Kinases / antagonists & inhibitors
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Adenosine Triphosphate / metabolism
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Arabidopsis / cytology*
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Arabidopsis / genetics
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Arabidopsis / metabolism
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / metabolism*
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DNA, Bacterial / genetics
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Flow Cytometry
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Gene Knockout Techniques
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Hypocotyl / cytology*
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Hypocotyl / genetics
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Hypocotyl / metabolism
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Membrane Proteins / genetics
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Membrane Proteins / metabolism
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Membrane Transport Proteins / genetics
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Membrane Transport Proteins / metabolism*
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Microscopy, Confocal
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Microscopy, Electron
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Mitochondria / metabolism
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Mitochondrial Membrane Transport Proteins / genetics
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Mitochondrial Membrane Transport Proteins / metabolism*
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Mitochondrial Membrane Transport Proteins / physiology
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Mitochondrial Precursor Protein Import Complex Proteins
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Mutagenesis, Insertional
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Phenotype
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Polymerase Chain Reaction
Substances
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Arabidopsis Proteins
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DNA, Bacterial
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Membrane Proteins
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Membrane Transport Proteins
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Mitochondrial Membrane Transport Proteins
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Mitochondrial Precursor Protein Import Complex Proteins
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T-DNA
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TIM50 protein, Arabidopsis
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Adenosine Triphosphate
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AMP-Activated Protein Kinases