Abstract
In eukaryotes, it is unknown whether mismatch repair (MMR) is temporally coupled to DNA replication and how strand-specific MMR is directed. We fused Saccharomyces cerevisiae MSH6 with cyclins to restrict the availability of the Msh2-Msh6 mismatch recognition complex to either S phase or G2/M phase of the cell cycle. The Msh6-S cyclin fusion was proficient for suppressing mutations at three loci that replicate at mid-S phase, whereas the Msh6-G2/M cyclin fusion was defective. However, the Msh6-G2/M cyclin fusion was functional for MMR at a very late-replicating region of the genome. In contrast, the heteroduplex rejection function of MMR during recombination was partially functional during both S phase and G2/M phase. These results indicate a temporal coupling of MMR, but not heteroduplex rejection, to DNA replication.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Cell Division
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Cyclin B / genetics
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Cyclin B / metabolism
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DNA Mismatch Repair*
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DNA Replication*
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DNA, Fungal / metabolism*
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism
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Frameshift Mutation
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G2 Phase
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Genes, Fungal
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Mutation
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Nucleic Acid Heteroduplexes / metabolism*
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Recombinant Fusion Proteins / metabolism
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Recombination, Genetic
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S Phase
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism*
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Saccharomyces cerevisiae / physiology
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism
Substances
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CLB2 protein, S cerevisiae
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CLB6 protein, S cerevisiae
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Cyclin B
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DNA, Fungal
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DNA-Binding Proteins
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G-T mismatch-binding protein
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Nucleic Acid Heteroduplexes
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Recombinant Fusion Proteins
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Saccharomyces cerevisiae Proteins