Xenopus paraxial protocadherin inhibits Wnt/β-catenin signalling via casein kinase 2β

EMBO Rep. 2012 Feb 1;13(2):129-34. doi: 10.1038/embor.2011.240.

Abstract

Xenopus paraxial protocadherin (PAPC) regulates cadherin-mediated cell adhesion and promotes the planar cell polarity (PCP) pathway. Here we report that PAPC functions in the Xenopus gastrula as an inhibitor of the Wnt/β-catenin pathway. The intracellular domain of PAPC interacts with casein kinase 2 beta (CK2β), which is part of the CK2 holoenzyme. The CK2α/β complex stimulates Wnt/β-catenin signalling, and the physical interaction of CK2β with PAPC antagonizes this activity. By this mechanism, PAPC restricts the expression of Wnt target genes during gastrulation. These experiments identify a novel function of protocadherins as regulators of the Wnt pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cadherins / chemistry
  • Cadherins / metabolism*
  • Casein Kinase II / metabolism*
  • Models, Biological
  • Protein Binding
  • Protein Structure, Tertiary
  • Protocadherins
  • Up-Regulation
  • Wnt Signaling Pathway*
  • Xenopus / embryology
  • Xenopus / metabolism*
  • Xenopus Proteins / chemistry
  • Xenopus Proteins / metabolism*

Substances

  • Cadherins
  • Pcdh8 protein, Xenopus
  • Protocadherins
  • Xenopus Proteins
  • Casein Kinase II