Complete genomic sequence analysis reveals a novel fabavirus infecting cucurbits in China

Arch Virol. 2012 Mar;157(3):597-600. doi: 10.1007/s00705-011-1202-x. Epub 2011 Dec 21.

Abstract

The complete genome sequence of a cucurbit-infecting fabavirus was determined. Sequence analysis revealed that it had a genomic organization typical of fabaviruses, with genome segment sizes of 5870 nt (RNA-1) and 3294 nt (RNA-2). It shared CP and Pro-Pol amino acid sequence identities of 52.0-58.9% with those of reported fabaviruses. ELISA and western blots gave no cross-reactions between this cucurbit virus and broad bean wilt viruses 1 and 2. Based on molecular and serological criteria for species demarcation in the genus Fabavirus, the virus represents a distinct species, for which the species name Cucurbit mild mosaic virus (CuMMV) is proposed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Viral / immunology
  • Blotting, Western
  • China
  • Cluster Analysis
  • Cross Reactions
  • Cucurbita / virology*
  • Enzyme-Linked Immunosorbent Assay
  • Fabavirus / classification
  • Fabavirus / genetics*
  • Fabavirus / immunology
  • Fabavirus / isolation & purification*
  • Genome, Viral*
  • Molecular Sequence Data
  • Phylogeny
  • RNA, Viral / genetics*
  • Sequence Analysis, DNA*
  • Sequence Homology, Amino Acid

Substances

  • Antibodies, Viral
  • RNA, Viral

Associated data

  • GENBANK/EU881936
  • GENBANK/EU881937