Hydration status has a major impact on hemodialysis (HD) patients. Overhydration is related to hypertension, pulmonary and peripheral edema, and other cardiovascular events; while dehydration is related to hypotension, and other severe ischemic symptoms. All result in increased morbidity and mortality. Bioimpedance has been newly developed to measure the amount of water in the body. Several predictive equations were used, taken from demographic and anthropometric data. The purpose of this study was to evaluate the body composition of HD patients and to propose a hydration index. We performed bioimpedance measures with the Tanita TBF-300 scale, which calculates Total Body Water (TBW). The tool was reliable, with good reproducibility. However, we found significant differences between weight variations (dW) and TBW variations (dTBW) during HD sessions. This paper proposes a hydration index (I=dW-dTBW), with the hypothesis that dry weight is reached when I=0, while I>0 or I<0 indicate overhydrated or dehydrated, respectively. In this study, the changes in the index corresponded to the variations in weight and hydration state. We conclude that impedancemetry is a currently available technique that can be used to estimate TBW in HD patients. Although the index has to be improved by complementary studies, it may be a good guide to assess the dry weight achieved.