Abstract
We have developed a method to analyze the substrate transport of ATP-binding cassette (ABC) transporters, which are associated with drug resistance in tumor cells. Our microfluidic method is well suited to the single-vesicle estimation of substrate transport and the rapid drug screening of ABC transporters. Using this method, we have demonstrated, for the first time, the analysis of substrate transport by a single transporter and performed drug-inhibition experiments in less than 3 h.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ATP Binding Cassette Transporter, Subfamily B
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ATP Binding Cassette Transporter, Subfamily B, Member 1 / antagonists & inhibitors*
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ATP Binding Cassette Transporter, Subfamily B, Member 1 / chemistry
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ATP Binding Cassette Transporter, Subfamily B, Member 1 / metabolism
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Cell Line, Tumor
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Drug Resistance, Neoplasm*
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Drug Screening Assays, Antitumor* / instrumentation
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Drug Screening Assays, Antitumor* / methods
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Humans
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Liposomes / chemistry
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Microfluidic Analytical Techniques* / instrumentation
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Microfluidic Analytical Techniques* / methods
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Neoplasms / chemistry
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Neoplasms / drug therapy
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Neoplasms / metabolism*
Substances
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ABCB1 protein, human
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ATP Binding Cassette Transporter, Subfamily B
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ATP Binding Cassette Transporter, Subfamily B, Member 1
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Liposomes