SOX7 is involved in aspirin-mediated growth inhibition of human colorectal cancer cells

World J Gastroenterol. 2011 Nov 28;17(44):4922-7. doi: 10.3748/wjg.v17.i44.4922.

Abstract

Aim: To confirm the role of sex-determining region Y-box 7 (Sox7) in aspirin-mediated growth inhibition of COX-independent human colorectal cancer cells.

Methods: The cell survival percentage was examined by MTT (Moto-nuclear cell direc cytotoxicity) assay. SOX7 expression was assessed by using reverse transcription-polymerase chain reaction and Western blotting. SB203580 was used to inhibit the p38MAPK signal pathway. SOX7 promoter activity was detected by Luciferase reporter assay.

Results: SOX7 was upregulated by aspirin and was involved in aspirin-mediated growth inhibition of SW480 human colorectal cancer cells. The p38MAPK pathway played a role in aspirin-induced SOX7 expression, during which the AP1 transcription factors c-Jun and c-Fos upregulated SOX7 promoter activities.

Results: SOX7 is upregulated by aspirin and is involved in aspirin-mediated growth inhibition of human colorectal cancer SW480 cells.

Keywords: Aspirin; Colorectal cancer; SB203580; SOX7; p38MAPK.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Inflammatory Agents, Non-Steroidal* / pharmacology
  • Anti-Inflammatory Agents, Non-Steroidal* / therapeutic use
  • Aspirin* / pharmacology
  • Aspirin* / therapeutic use
  • Cell Line
  • Colorectal Neoplasms / drug therapy*
  • Colorectal Neoplasms / pathology
  • Colorectal Neoplasms / physiopathology*
  • Cyclooxygenase 2 / metabolism
  • Cyclooxygenase 2 Inhibitors* / pharmacology
  • Cyclooxygenase 2 Inhibitors* / therapeutic use
  • Gene Expression Regulation / drug effects
  • Humans
  • MAP Kinase Signaling System / drug effects
  • SOXF Transcription Factors / genetics
  • SOXF Transcription Factors / metabolism*
  • p38 Mitogen-Activated Protein Kinases / metabolism

Substances

  • Anti-Inflammatory Agents, Non-Steroidal
  • Cyclooxygenase 2 Inhibitors
  • SOX7 protein, human
  • SOXF Transcription Factors
  • Cyclooxygenase 2
  • PTGS2 protein, human
  • p38 Mitogen-Activated Protein Kinases
  • Aspirin