[Screening interaction protein of Schistosoma japonicum gynecophoral canal protein by phage display]

Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2011 Jun;23(3):279-84.
[Article in Chinese]

Abstract

Objective: To search the interaction protein of Schistosoma japonicum gynecophoral canal protein (SjGCP).

Methods: The recombinant rSjGCP was used as a target to search the T7 phage display cDNA library from 44-day adult Schistosoma japonicum.

Results: A total of 70 ESTs were obtained. The bioinformatics analyses to the screening results revealed that 5 interaction proteins or peptides of SjGCP were found. CONCLUSION; Interaction proteins or peptides of SjGCP are successfully obtained through screening the T7 phage display library.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Expressed Sequence Tags
  • Female
  • Glycoproteins / genetics*
  • Glycoproteins / metabolism*
  • Helminth Proteins / genetics*
  • Helminth Proteins / metabolism*
  • Male
  • Molecular Sequence Data
  • Peptide Library
  • Protein Binding
  • Schistosoma japonicum / genetics
  • Schistosoma japonicum / metabolism*

Substances

  • Glycoproteins
  • Helminth Proteins
  • Peptide Library
  • gynecophoral canal protein, 86-kDa