A new and sensitive catalytic resonance scattering spectral assay for the detection of laccase using guaiacol as substrate

Caina Jiang; Shaoming Ling; Pengfei Wang; Aihui Liang; Bin Chen; Guiqing Wen; Zhiliang Jiang

doi:10.1002/bio.1259

A new and sensitive catalytic resonance scattering spectral assay for the detection of laccase using guaiacol as substrate

Luminescence. 2011 Nov-Dec;26(6):500-5. doi: 10.1002/bio.1259. Epub 2010 Dec 13.

Authors

Caina Jiang¹, Shaoming Ling, Pengfei Wang, Aihui Liang, Bin Chen, Guiqing Wen, Zhiliang Jiang

Affiliation

¹ Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, School of Environment and Resource, Guangxi Normal University, Guilin 541004, China.

PMID: 22162451
DOI: 10.1002/bio.1259

Abstract

In pH 4.0 succinic acid-sodium hydroxide buffer solution, laccase catalyzed the oxidization of guaiacol substrate to form red particles, which exhibited a strong resonance scattering (RS) peak at 590 nm. Under the chosen conditions, as the laccase increased, the RS intensity (ΔI) increased linearly. The ΔI was proportional to laccase activity in the range of 0.10-1.2 U/mL, with a regression equation of ΔI = 734.0 U(laccase) - 9.7, and a detection limit of 0.05 U/mL. This RS method was applied to the detection of laccase activity in real samples, and the results were agreement with those from spectrophotometry.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Catalysis
Guaiacol / metabolism*
Hydrogen-Ion Concentration
Laccase / analysis*
Laccase / metabolism
Microscopy, Electron, Scanning
Spectrometry, Fluorescence
Substrate Specificity

Substances

Guaiacol
Laccase