The concentrations of nine mineral elements were determined by atomic absorption spectroscopy in Gentiana rigescens from three zones (middle, southeast, and northwest zones) of Yunnan province in China. The average concentrations of mineral elements were found in the order K>Ca>Mg>Fe>Na>Zn>Cu>Cr>Se. Samples collected from the middle zone of Yunnan were enriched in Na (242.24 ± 50.80 mg/kg) and Mg (959.78 ± 54.60 mg/kg). However, materials from the southeast zone were particularly enriched in Ca (3,448.70 ± 749.82 mg/kg), but depleted in Se. Whereas those from the northwest zone were plentiful of K (2,746.89 ± 84.84 mg/kg), Fe (923.06 ± 127.52 mg/kg), Zn (261.38 ± 53.05 mg/kg), Cu (110.08 ± 46.23 mg/kg), and Se (53.99 ± 22.38 mg/kg).