Preparation of a novel molecularly imprinted polymer by the sol-gel process for sensing creatinine

Abstract

A novel molecularly imprinted polymer (MIP) was prepared and used as an artificial receptor for creatinine (Cre). A sol-gel process was used to prepare the MIP. Tetraethoxysilane (TEOS) was employed as the crosslinker for the formation of a silica matrix for the MIP. Aluminum ion (Al(3+)) was chosen as the dopant to generate Lewis acid sites in the silica matrix for interactions with Cre. Through the sol-gel process, a polymeric matrix with memory sites for Cre was obtained, and this is mentioned here as the molecularly imprinted polymer for creatinine (MIP(Cre)). The imprinting efficiency of MIP(Cre) was evaluated by contrasting the adsorbed amount of Cre by MIP(Cre) with that by the corresponding non-imprinted polymer (NIP). Creatine (Cn), N-hydroxysuccinimide (NHS), and L-tyrosine (L-tyr) were selected as interferences to study the selectivity of the MIP(Cre). The interference studies were also conducted using binary mixtures, such as Cre/Cn, Cre/NHS, and Cre/L-tyr. All these studies reveal that the MIP(Cre) possess a remarkable affinity for Cre. The crucial role of Al(3+) in this system is discussed in detail. Furthermore, the effects of concentrations of Al(3+) and TEOS on the adsorbed amount of Cre by MIP(Cre) were also investigated.