Transcriptome analysis of an oil-rich race A strain of Botryococcus braunii (BOT-88-2) by de novo assembly of pyrosequencing cDNA reads

Masato Baba; Motohide Ioki; Nobuyoshi Nakajima; Yoshihiro Shiraiwa; Makoto M Watanabe

doi:10.1016/j.biortech.2011.10.033

Transcriptome analysis of an oil-rich race A strain of Botryococcus braunii (BOT-88-2) by de novo assembly of pyrosequencing cDNA reads

Bioresour Technol. 2012 Apr:109:282-6. doi: 10.1016/j.biortech.2011.10.033. Epub 2011 Oct 19.

Authors

Masato Baba¹, Motohide Ioki, Nobuyoshi Nakajima, Yoshihiro Shiraiwa, Makoto M Watanabe

Affiliation

¹ Graduate School of Life and Environment Sciences, University of Tsukuba, Tennoudai 1-1-1, Tsukuba, Ibaraki 305-8572, Japan.

PMID: 22137751
DOI: 10.1016/j.biortech.2011.10.033

Abstract

To gain genetic information of oil-producing algae Botryococcus braunii, a novel dataset of 185,936 complementary DNA (cDNA) reads was obtained via pyrosequencing for the representative race A strain (strain BOT-88-2) exhibiting high oil productivity. The cDNA reads were assembled to retrieve 29,038 non-redundant sequences and 964 of them were successfully annotated based on similarity to database sequences. The transcriptome data embraced candidate genes for majority of enzymes involved in the biosynthesis of unsaturated very long-chain fatty acids. The transcriptome dataset has been deposited in the GenBank/EMBL/DDBJ database.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chlorophyta / enzymology
Chlorophyta / genetics*
DNA, Complementary / genetics*
Databases, Genetic
Expressed Sequence Tags
Fatty Acids / metabolism
Gene Expression Profiling / methods*
Plant Oils / metabolism*
Sequence Analysis, DNA / methods*
Temperature*

Substances

DNA, Complementary
Fatty Acids
Plant Oils