Techniques for the study of apoptosis in bone

Methods Mol Biol. 2012:816:335-49. doi: 10.1007/978-1-61779-415-5_22.

Abstract

There has been great interest in the identifying the mechanisms by which apoptosis is regulated in bone over recent years and in the biological role that this process plays in bone metabolism and bone disease. Here, we describe several methods for the detection of apoptosis in bone sections and in bone cell cultures.

MeSH terms

  • Animals
  • Apoptosis*
  • Bone and Bones / cytology*
  • Bone and Bones / metabolism
  • Caspases / metabolism
  • Cell Culture Techniques
  • Cell Survival
  • DNA Fragmentation
  • Humans
  • L-Lactate Dehydrogenase / metabolism
  • Osteocytes / cytology
  • Osteocytes / metabolism
  • Staining and Labeling / methods
  • Tolonium Chloride / analysis

Substances

  • Tolonium Chloride
  • L-Lactate Dehydrogenase
  • Caspases