Site-specific conjugation of ScFvs antibodies to nanoparticles by bioorthogonal strain-promoted alkyne-nitrone cycloaddition

Angew Chem Int Ed Engl. 2012 Jan 9;51(2):496-9. doi: 10.1002/anie.201106775. Epub 2011 Nov 25.

Abstract

Particularly suitable: An N-terminal serine mutant of anti-HER2 scFv antibody was conjugated to polymer-coated magnetofluorescent nanoparticles by strain-promoted alkyne-nitrone cycloaddition. The resulting nanoparticles (see scheme) proved effective in targeting and labeling HER2-positive breast cancer cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkynes / chemistry
  • Cell Line, Tumor
  • Cyclization
  • Humans
  • Mutation
  • Nanoparticles / chemistry*
  • Nitrogen Oxides / chemistry*
  • Receptor, ErbB-2 / analysis*
  • Receptor, ErbB-2 / immunology*
  • Single-Chain Antibodies / chemistry*
  • Single-Chain Antibodies / genetics
  • Single-Chain Antibodies / immunology*

Substances

  • Alkynes
  • Nitrogen Oxides
  • Single-Chain Antibodies
  • nitrones
  • Receptor, ErbB-2