Observation of the influence oftestosterone (0.7 mg/kg) on the activity ofglycosidases and proteinases, which function in the chime and the mucous coat of the sterlet intestine (Acipenser ruthenus), revealed a decrease in enzyme activity of both chains as against that one typical for intact individuals. The activity of the investigated enzymes changes in both the experimental and control groups offish. Nevertheless, they have unlike dynamics of this activity. In a number of cases, we indicated a significant increase in enzyme activity of both chains in comparison to the control group. We also observed an increase in activity of chime glycosidases, which was significant on the 21st day as compared to intact fish.