Abstract
We studied 123 samples from adult chronic HIV patients initiating HAART from various centers around a newly established clinical trial site in Pretoria. Each sample was sequenced in at least one structural gene (pol, gag, and env) or functional gene (vif, vpr, and vpu). A subset of 25 samples was subjected to near full-genome analysis. All samples were HIV-1 subtype C. Highly conserved regions within the gene sequences were observed. Overall, the gag and vif sequences showed closer similarity followed by the env, vpr, pol, and vpu. The env gene was the most difficult to sequence, resulting in only 31 sequences from 40 samples; of these, 25 were predicted to be R5 coreceptor tropic, while 6 were X4 tropic. The study asserted the predominance of HIV-1 subtype C within the catchment population.
Publication types
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Multicenter Study
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Research Support, Non-U.S. Gov't
MeSH terms
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AIDS Vaccines*
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Acquired Immunodeficiency Syndrome / drug therapy
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Acquired Immunodeficiency Syndrome / epidemiology*
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Acquired Immunodeficiency Syndrome / prevention & control
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Amino Acid Sequence
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Antiretroviral Therapy, Highly Active
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Female
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Gene Amplification
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Genes, env / genetics*
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Genes, gag / genetics*
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Genes, pol / genetics*
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Genome, Viral
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HIV-1 / genetics*
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Human Immunodeficiency Virus Proteins / genetics
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Humans
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Male
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Molecular Sequence Data
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Phylogeny*
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Polymerase Chain Reaction
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Sequence Alignment
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Sequence Analysis, Protein
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South Africa / epidemiology
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Viral Regulatory and Accessory Proteins / genetics
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vif Gene Products, Human Immunodeficiency Virus / genetics
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vpr Gene Products, Human Immunodeficiency Virus / genetics
Substances
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AIDS Vaccines
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Human Immunodeficiency Virus Proteins
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Viral Regulatory and Accessory Proteins
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vif Gene Products, Human Immunodeficiency Virus
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vpr Gene Products, Human Immunodeficiency Virus
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vpu protein, Human immunodeficiency virus 1