An improved rapid method for the preparation of D-rhamnose

Olga N Smol'kina; Natalya S Shishonkova; Yulia P Fedonenko; Evelina L Zdorovenko; Svetlana A Konnova; Vladimir V Ignatov

doi:10.1016/j.carres.2011.10.030

An improved rapid method for the preparation of D-rhamnose

Carbohydr Res. 2012 Jan 10;347(1):161-3. doi: 10.1016/j.carres.2011.10.030. Epub 2011 Oct 28.

Authors

Olga N Smol'kina¹, Natalya S Shishonkova, Yulia P Fedonenko, Evelina L Zdorovenko, Svetlana A Konnova, Vladimir V Ignatov

Affiliation

¹ Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, Saratov 410049, Russia. room308@ibppm.sgu.ru

PMID: 22099380
DOI: 10.1016/j.carres.2011.10.030

Abstract

A method is developed for the preparation of D-rhamnose from an O-polysaccharide (OPS) isolated by mild acid hydrolysis of Azospirillum brasilense SR75 cell mass. After the OPS hydrolysis, D-rhamnose was recovered by gel-permeation chromatography on Toyopearl TSK HW-40 and was crystallized. The sugar activity was demonstrated immunochemically. The advantages of the method are that it expedites and simplifies the extraction of D-rhamnose and increases its yield.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Azospirillum brasilense / chemistry
Chemical Fractionation / methods*
Chromatography, Gel
O Antigens / chemistry
Rhamnose / isolation & purification*
Time Factors

Substances

O Antigens
Rhamnose