Detection of foot-and-mouth disease virus RNA by reverse transcription loop-mediated isothermal amplification

Hao-tai Chen; Jie Zhang; Yong-sheng Liu; Xiang-tao Liu

doi:10.1186/1743-422X-8-510

Detection of foot-and-mouth disease virus RNA by reverse transcription loop-mediated isothermal amplification

Virol J. 2011 Nov 9:8:510. doi: 10.1186/1743-422X-8-510.

Authors

Hao-tai Chen¹, Jie Zhang, Yong-sheng Liu, Xiang-tao Liu

Affiliation

¹ State Key Laboratory of Veterinary Etiologic Biology, National Foot-and-Mouth Disease Reference Laboratory of China, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, Gansu, P.R. China.

Abstract

A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for foot-and-mouth disease virus (FMDV) RNA. The amplification was able to finish in 45 min under isothermal condition at 64°C by employing a set of four primers targeting FMDV 2B. The assay showed higher sensitivity than RT-PCR. No cross reactivity was observed from other RNA viruses including classical swine fever virus, swine vesicular disease, porcine reproductive and respiratory syndrome virus, Japanese encephalitis virus. Furthermore, the assay correctly detected 84 FMDV positive samples but not 65 FMDV negative specimens. The result indicated the potential usefulness of the technique as a simple and rapid procedure for the detection of FMDV infection.

Publication types

Comparative Study
Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cross Reactions
Foot-and-Mouth Disease / diagnosis*
Foot-and-Mouth Disease / virology
Foot-and-Mouth Disease Virus / genetics
Foot-and-Mouth Disease Virus / isolation & purification*
Molecular Diagnostic Techniques / methods*
Nucleic Acid Amplification Techniques / methods*
RNA, Viral / genetics
RNA, Viral / isolation & purification*
Sensitivity and Specificity
Temperature
Time Factors
Veterinary Medicine / methods
Virology / methods*

Substances

RNA, Viral