Abstract
We determined the prevalence of gyrA mutations conferring fluoroquinolone resistance in 97 Helicobacter pylori isolates collected in France from 2007 to 2010. Ninety-four harbored one or two mutations already found in the quinolone resistance determining region (QRDR) of gyrA (for T87I, n = 23; for N87K, n = 32; for D91N, n = 30; for D91G, n = 7; for D91Y, n = 6), 2 harbored a mutation never previously described (D91H and A88P), and one strain was resistant (ciprofloxacin MIC of 8 mg/liter) without a detected mutation conferring this resistance in gyrA or gyrB genes.
MeSH terms
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Amino Acid Substitution
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Anti-Bacterial Agents / administration & dosage
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Anti-Bacterial Agents / therapeutic use
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Bacterial Proteins / genetics
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Bacterial Typing Techniques
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Ciprofloxacin / administration & dosage
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Ciprofloxacin / therapeutic use*
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DNA Gyrase / genetics*
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DNA Mutational Analysis
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DNA, Bacterial
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Drug Resistance, Bacterial
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Fluoroquinolones / administration & dosage
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Fluoroquinolones / therapeutic use
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France
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Helicobacter Infections / drug therapy*
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Helicobacter Infections / microbiology
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Helicobacter pylori / drug effects
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Helicobacter pylori / genetics*
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Helicobacter pylori / isolation & purification
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Humans
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Microbial Sensitivity Tests
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Mutation
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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DNA, Bacterial
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Fluoroquinolones
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Ciprofloxacin
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DNA Gyrase